Nematode screening

The South Australian Research and Development Institute (SARDI) Nematology group offers screening services for:

  • cereal cyst nematode
  • pratylenchus
  • stem nematode.

Our services are on a fee-for-service basis.

We also conduct general nematode soil testing. Please see Horticulture Pathology.

Cereal cyst nematode

We provide two types of assays for screening wheat, barley, oats and triticale lines. Both screen for resistance to cereal cyst nematode (CCN).

They are not destructive tests. This means we have the capacity to tag and retain resistant seed producing plants.

Large scale ‘POTS’  assay

We recommend this assay to determine the resistance rating of early generation breeding material.

You can use the screening results to indicate lines susceptible or resistant to CCN.

About this service:

  • the high-throughput screening service is an outdoor assay
  • plants are sown into seedling tubes containing CCN cyst infested soil
  • we sow plants in June and assess in October / November.

Small scale controlled environment room (CER) assay

We recommend that advanced lines be screened using the CER test (prior to release).

This will allow you to confirm or identify their CCN resistance rating.

About this service:

  • this screening service is a growth room assay
  • we inoculate seedlings with CCN larvae and assess them 10 weeks after inoculation
  • the CER is more labour intensive than the POTS
  • it is also more accurate; we assess all CCN from the root system and the soil surrounding the plant.

Access our CCN services

Please contact John Lewis if you:

  • require further information about either of the CCN screening services
  • want to submit lines for evaluation
  • need advice regarding CCN management experiments.

Contact

John Lewis
Phone: (08) 8303 9394
Mobile: 0401 711 758
Email: john.lewis@sa.gov.au

Pratylenchus

Our screening services assess resistance to the root lesion nematodes Pratylenchus neglectus and P. thornei.

About this service:

  • the screening test is a glasshouse assay
  • plant seedlings are inoculated with either P. neglectus or P. thornei and nematode numbers assessed from root systems after 8 weeks.

This assay is destructive, meaning we cannot keep seeds from individual plants.

Please contact Danuta Pounsett if you:

  • require further information about our screening services
  • want to submit lines for evaluation.

Contact

Danuta Pounsett
Phone: (08) 8303 9437
Email: danuta.pounsett@sa.gov.au

Page Last Reviewed: 06 Feb 2015
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